CarboClip is a 37.2 kDa recombinant peptide-N-Glycosidase F (PNGase F), from Elizabethkingia sp, overexpressed in E. coli that cleaves between the GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins.
The absence of glycerol makes this enzyme compatible with Mass Spectrometry applications.
CarboClip is provided glycerol-free as a lyophilized powder containing Tris-HCl, NaCl2, and Na2EDTA. Stored at -80 °C it has a shelf life of > 1year,
In lyophilized form it can be can be shipped at room temperature. For larger volumes (>100 vials, 10.000Units) we offer customized enzyme aliquotation/packaging.
One CarboClip unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 10 µg of denatured RNase B in 1 minute at 37°C in a total reaction volume of 10 µl.
download instructions for use here