Glycosylation Of Extracellular Vesicles: Current Knowledge, Tools And Clinical Perspectives, Charles Williams, Felix Royo, Oier Aizpurua-Olaizola, Raquel Pazos, Geert-Jan Boons, Niels-Christian Reichardt and Juan M Falcon-Perez, J. Extracell. Vesicles 2018, article accepted


Identification of dominant anti-glycan IgE responses in school-children by glycan microarrayAbena S. Amoah, Elias K. Asuming-Brempong, Benedicta B. Obeng, Serge A. Versteeg, Irene A. Larbi, Yvonne Aryeetey, Thomas A.E. Platts-Mills, Adriano Mari, Katarzyna Brzezicka, Ben A. Gyan, Mohamed Mutocheluh, Daniel A. Boakye, Niels-Christian Reichardt, Ronald van Ree, Cornelis H. Hokke, Angela van Diepen, Maria Yazdanbakhsh,  J. Allergy Clin. Immunol. 2017, article in press

Analysis of Defective Protein Ubiquitylation Associated to Adriamycin Resistant Cells Valérie Lang, Fabienne Aillet, Wendy Xolalpa, Sonia Serna, Laurie Ceccato,
Rosa G. Lopez-Reyes, Maria Paz Lopez-Mato, Radosław Januchowski, Niels-
Christian Reichardt, Manuel S. Rodriguez, Cell Cycle, 2017, early view online version

Lectin-Array Blotting, Raquel Pazos, Juan Echevarria, Alvaro Hernandez, Niels-Christian Reichardt, Curr. Protocols in Cell Biol. 2017, DOI: 10.1002/cpcb.20

NMR and Molecular Recognition of N-Glycans: Remote Modifications of the Saccharide Chain Modulate Binding Features,  Ana Gimeno, Niels-Christian Reichardt, Francisco Javier Cañada, Lukas Perkams, Carlo Unverzagt, Jesus Jimenez-Barbero, and Ana Arda,  ACS Chem. Biol., 2017, 12 (4), pp 1104–1112

Specific anti-glycan antibodies are sustained during and after parasite clearance in Schistosoma japonicum-infected rhesus macaques Y.Y. Michelle Yang, Xiao Hong Li, Katarzyna Brzezicka, Niels-Christian Reichardt, R. Alan Wilson, Angela van Diepen and Cornelis H. Hokke,  PLoS Neglected Tropical Diseases 2017,

Fluoroacetamide Moieties as NMR Probes for molecular recognition of GlcNAc-containing sugars: Modulation of the CH-π Stacking Interactions by Different Fluorination Patterns, Luca Unione, Maria Alcalá, Begoña Echeverria, Sonia Serna, Ana Ardá, Antonio Franconetti, F. Javier Cañada, Tammo Diercks, Niels Reichardt, Jesús Jiménez-Barbero, Chemistry,-Eur. J. .2017, DOI: 10.1002/chem.201605573


Carbohydrate-Binding Non-Peptidic Pradimicins for the Treatment of Acute Sleeping Sickness in Murine Models, Castillo-Acosta VM, Ruiz-Pérez LM, Etxebarria J, Reichardt NC, Navarro M, Igarashi Y, et al. (2016)  PLoS Pathog 12(9): e1005851. DOI:10.1371/journal.ppat.1005851

Opportunities for Glyconanomaterials in Personalized Medicine, Reichardt, N.-C.; Martin-Lomas, M. ; Penades, S.; Chem. Commun., 2016, 52, 13430 – 13439

Efficient monitoring of protein ubiquitylation levels using TUBEs-based microarraysSerna, S., Xolalpa, W., Lang, V., Aillet, F., England, P., Reichardt, N., & Rodriguez, M. S.  FEBS letters, 2016,  590,(16), 2748–2756

Influence of Core β-1,2-xylosylation on Glycoprotein Recognition by Murine C-type Lectin Receptors and its Impact on Dendritic Cell Targeting; Brzezicka K.;  Vogel U., Serna S., Johannssen T., Lepenies B.,  Reichardt N.C., ACS Chem. Biol., 2016, 11 (8), pp 2347–2356

Methods for the absolute quantification of N-glycan biomarkers. Etxebarria J. and Reichardt N.C.; Biochim. Biophys Acta, 1860,8, 1676–1687 (2016)


Chemo-Enzymatic Synthesis of 13C Labeled Complex N-Glycans As Internal Standards for the Absolute Glycan Quantification by Mass Spectrometry. Echeverria B., Etxebarria J., Ruiz N., Hernandez A., Calvo J., Haberger M, Reusch D. and Reichardt N.C.; Anal. Chem. , 87 (22), 11460–11467 (2015)

On the molecular interaction between albumin and ibuprofen: an AFM and QCM-D study. Eleta-Lopez A., Etxebarria J., Reichardt N., Georgieva R., Bäumler H. and Toca-Herrera J.L.; Colloid Surface. B 134, 355-362 (2015).

Monitoring Glycan-Protein Interactions by NMR Spectroscopic Analysis: A Simple Chemical Tag That Mimics Natural CH-π Interactions. Calle L.P., Echeverría B., Franconetti A., Serna S., Fernández-Alonso M.C., Diercks T., Cañada J., Ardá A., Reichardt N.C. and Jiménez-Barbero J.; Chem. Eur. J., 21, 11408–11416 (2015).

Algal lectin binding to core (α1-6) fucosylated N-glycans: Structural basis for specificity and production of recombinant protein. Do Nascimento A.S.F., Serna S., Beloqui A., Arda A., Sampaio A.H., Walcher J., Ott D., Unverzagt C., Reichardt N.C., Jimenez-Barbero J., Nascimento K.S., Imberty A., Cavada B.S. and Varrot A.; Glycobiology 25, 607 – 616 (2015).

Synthesis and Microarray-Assisted Binding Studies of Core Xylose and Fucose Containing N-Glycans. Brzezicka K., Echeverria B., Serna S.,Van Diepen A., Hokke C.H. and Reichardt N.C.; ACS Chem. Biol. 10, 1290 – 1302 (2015).

Solid-phase assembly of glycosaminoglycan oligosaccharide precursors. Guedes N., Kopitzki S., Echeverría B., Pazos R., Elosegui E., Calvo J. and Reichardt N.C.; RSC Adv. 5, 9325 – 9327 (2015).

Nanostructured Indium Tin Oxide Slides for Small-Molecule Profiling and Imaging Mass Spectrometry of Metabolites by Surface-Assisted Laser Desorption Ionization MS. Lopez de Laorden C., Beloqui A., Yate L., Calvo J., Puigivila M., Llop J. and Reichardt N.C.; Anal. Chem. 87, 431-440 (2015).


Importance of the polarity of the glycosaminoglycan chain on the interaction with FGF-1. Muñoz-García J.C., García-Jiménez M.J., Carrero P., Canales A., Jiménez-Barbero J., Martín-Lomas M., Imberty A., de Paz J. L., Angulo J., Lortat-Jacob H. and Nieto P.M.; Glycobiology 24, 1004-1009 (2014).

Microarray-Based Identification of Lectins for the Purification of Human Urinary Extracellular Vesicles Directly from Urine Samples. Echevarria J., Royo F., Pazos R., Salazar L., Falcon-Perez J.M. and Reichardt N.C.; ChemBioChem 15, 1621 – 1626 (2014).

Cross-platform comparison of glycan microarray formats. Wang L., Cummings R.D., Smith D.F., Huflejt M., Campbell C.T. Gildersleeve J.C. Gerlach J.Q., Kilcoyne M., Joshi L., Serna S., Reichardt N.C., Parera Pera N., Pieters R.J., Eng W. and Mahal L.K.; Glycobiology 24, 507 – 517 (2014).

Nanostructured weathering steel for matrix-free laser desorption ionisation mass spectrometry and imaging of metabolites, drugs and complex glycans. Etxebarria J., Calvo J. and Reichardt N.C.; Analyst 139, 2873 – 2883 (2014).

Biological Evaluation of Multivalent Lewis X-MGL-1 Interactions. Eriksson M., Serna S., Maglinao M., Schlegel M.K., Seeberger P.H., Reichardt N.C. and Lepenies B.; ChemBioChem 15, 844 – 851 (2014).


Heparin Modulates the Mitogenic Activity of Fibroblast Growth Factor by Inducing Dimerization of its Receptor. A 3D View by Using NMR. Nieto L., Canales A., Fernández I.S., Santillana E., González-Corrochano R., Redondo-Horcajo M., Cañada F.J., Nieto P., Martín-Lomas M., Giménez-Gallego G. and Jiménez-Barbero J.; ChemBioChem 14, 1732-1744 (2013).

Towards the Solid-Phase Synthesis of Heparan Sulfate Oligosaccharides: Evaluation of Iduronic Acid and Idose Building Blocks. Guedes N., Czechura P., Echeverria B., Ruiz A., Michelena O., Martin-Lomas M. and Reichardt N.C.; J. Org. Chem. 78, 6911-6934 (2013).

Array-assisted characterisation of a fucosyltransferase required for the biosynthesis of complex core modifications of nematode N-glycans. Yan S., Serna S., Reichardt N.C, Paschinger K. and Wilson I. B. H.; J. Biol. Chem 288, 21015-21028 (2013).

Analysis of Microarrays by MALDI-TOF MS. Beloqui A., Calvo J., Serna S., Yan S., Wilson I. B. H, Martin-Lomas M. and Reichardt N.C.; Angew. Chem. Int. Ed. 52, 7477 -7481 (2013).

Glyconanotechnology. Reichardt N.C., Martín-Lomas M. and Penadés S.; Chem. Soc. Rev. 42, 4358-4376 (2013).

Profiling Glycosyltransferase Activities by Tritium Imaging of Glycan Microarrays . Serna S., Hokke C.H., Weissenborn M., Flitsch S., Martin-Lomas M. and Reichardt N.C.; ChemBioChem 14, 862-869 (2013).

Three-Dimensional Arrays Using GlycoPEG Tags: Glycan Synthesis, Purification and Immobilisation. Etxebarria J., Serna S., Beloqui A., Martin-Lomas M. and Reichardt N.C.; Chem. Eur. J. 19, 4776-4785 (2013).


Effect of the Substituents of the Neighboring Ring in the Conformational Equilibrium of Iduronate in Heparin-like Trisaccharides. Muñoz-Garcia J.C., Lopez-Prados J., Angulo J., Diaz-Contreras I., Reichardt N., de Paz J.L., Martin-Lomas M. and Nieto P.M.; Chem. Eur. J. 18, 16319-16331 (2012).

Lectin-Array Blotting: Profiling Protein Glycosylation in Complex Mixtures. Etxebarria J., Calvo J., Martin-Lomas M. and Reichardt N.C; ACS Chem. Biol. 7, 1729-1737 (2012).

A surface-based mass spectrometry method for screening glycosidase specificity in environmental samples. Beloqui A., Sanchez-Ruiz A.,Martin-Lomas M. and Reichardt N.C.; Chem. Commun. 48, 1701-1703 (2012).


Synthesis of 1,3,4,6-tetra-O-acetyl-2-azido-2-deoxy-α,β-D-glucopyranose and 2-azido-4,6-benzylidene-2-deoxy-α,β-D-glucopyranose. Ojeda R. , de Paz J. L., Lucas R., Reichardt N.-C., Liu L. and Martin-Lomas M.; Carbohydrate Chemistry. Proven Synthetic Methods (P. Kovac, Ed.) CRC Press 1, 205-211 (2011).

Fucosyltransferases as Synthetic Tools: Glycan Array Based Substrate Selection and Core Fucosylation of Synthetic N-Glycans. Serna S., Yan S., Martin-Lomas M., Wilson I.B.H and Reichardt N.C.; J. Am. Chem. Soc. 133, 16495-16502 (2011).

Experimental observations on the regioselectivity of glycosylation of a 4,6-diol system in the β-D-mannopyranosyl unit of a N-glycan pentasaccharide core structure. Ruiz N., Ferreira S., Padro D., Reichardt N.C., Martín-Lomas M.; Carbohyd. Res. 346, 1581-1591 (2011).

MALDI-TOF Mass Spectrometric Analysis of Enzyme Activity and Lectin Trapping on an Array of N-Glycans. Sanchez-Ruiz A., Serna S., Ruiz N., Martin-Lomas M., and Reichardt N.C.; Angew. Chem. Int. Ed. 50, 1801-1804 (2011).

A New Linker for Solid-Phase Synthesis of Heparan Sulfate Precursors by Sequential Assembly of Monosaccharide Building Blocks. Czechura P., Guedes N., Kopitzki S., Vazquez N., Martin-Lomas M. and Reichardt N.C.; Chem. Commun. 47, 8 2390 – 2392 (2011).


Construction of N-Glycan Microarrays by Using Modular Synthesis and On-Chip Nanoscale Enzymatic Glycosylation. Serna S., Etxebarria J., Ruiz N., Martin-Lomas M. and Reichardt N.C.; Chem. Eur. J. 16, 13163 – 13175 (2010).


Synthesis of a core trisaccharide building block for the assembly of N-glycan neoconjugates. Serna S., Kardak B., Reichardt N.-C. and Martín-Lomas M.; Tetrahedron-Asymmetry 20, 851-856 (2009).