A new review from our lab on absolute glycan quantification has just been published in BBA-General subjects. Methods for the absolute quantification of N-glycan biomarkers, Etxebarria J. and Reichardt N.C.; Bioachim. Biophys Acta 0, http://dx.doi.org/10.1016/j.bbagen.2016.03.003 (2016).
Glycan are promising biomarkers in precision medicine but require validation in large trials.
The large natural variability of glycan markers is mainly due to hereditary causes. Most current methods for glycan analysis are not suitable for clinical diagnostics.
Absolute quantification of glycan markers by mass spectrometry can be achieved employing heavy-isoptope labeled standards.
Internal standards can improve assay robustness, method transfer between labs and aid in patient stratification.